Why pH = pKa + log([A⁻]/[HA]) is all you need — pick the right buffer near its pKa and prep it right the first time.
Calculate the conjugate-acid and conjugate-base concentrations to mix a Phosphate buffer at your target pH and total molarity — with the working shown.
Calculate the conjugate-acid and conjugate-base concentrations to mix a Tris buffer at your target pH and total molarity — with the working shown.
Calculate the conjugate-acid and conjugate-base concentrations to mix a HEPES buffer at your target pH and total molarity — with the working shown.
Calculate the conjugate-acid and conjugate-base concentrations to mix a Acetate buffer at your target pH and total molarity — with the working shown.
Calculate the conjugate-acid and conjugate-base concentrations to mix a MOPS buffer at your target pH and total molarity — with the working shown.
Calculate the conjugate-acid and conjugate-base concentrations to mix a MES buffer at your target pH and total molarity — with the working shown.
Dilute a concentrated Tris stock to your working concentration — get the µL of stock and the diluent volume.
Dilute a 0.5 M EDTA stock to a working concentration for buffers and lysis mixes.
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