The one formula behind every working dilution — plus serial dilutions and how to hit an exact molarity from a stock.
Solve any dilution: enter stock concentration, target concentration and final volume to get the stock volume to pipette and the diluent to add.
Dilute a concentrated Tris stock to your working concentration — get the µL of stock and the diluent volume.
Dilute a 0.5 M EDTA stock to a working concentration for buffers and lysis mixes.
Dilute a 5 M NaCl stock to the salt concentration your protocol needs.
Dilute a 1 M MgCl₂ stock to the final mM for PCR or enzyme reactions.
Dilute concentrated or stock HCl to a working molarity — always add acid to water.
Calculate the molarity (mol/L) of a Sodium Chloride (NaCl) solution from the mass you weighed out and your final volume — shows the working and the millimolar value.
Calculate the molarity (mol/L) of a Potassium Chloride (KCl) solution from the mass you weighed out and your final volume — shows the working and the millimolar value.
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