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RCF vs RPM: Your Spin Speed Is Probably Wrong

Two rotors at the same RPM spin cells at very different g — convert RCF↔RPM with your rotor radius and protect your pellet.

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1 / 8

RCF → RPM Calculator (pelleting mammalian cells in a microfuge)

Convert a target relative centrifugal force (×g) into the rotor speed (RPM) you must dial in for pelleting mammalian cells in a microfuge, from your rotor radius.

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RPM → RCF Calculator (pelleting mammalian cells in a microfuge)

Convert centrifuge speed (RPM) to relative centrifugal force (×g) for pelleting mammalian cells in a microfuge, using your rotor radius. Always report ×g, not RPM, for reproducibility.

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3 / 8

RCF → RPM Calculator (a plasmid miniprep spin)

Convert a target relative centrifugal force (×g) into the rotor speed (RPM) you must dial in for a plasmid miniprep spin, from your rotor radius.

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4 / 8

RPM → RCF Calculator (DNA ethanol precipitation)

Convert centrifuge speed (RPM) to relative centrifugal force (×g) for DNA ethanol precipitation, using your rotor radius. Always report ×g, not RPM, for reproducibility.

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5 / 8

RPM → RCF Calculator (pelleting bacteria)

Convert centrifuge speed (RPM) to relative centrifugal force (×g) for pelleting bacteria, using your rotor radius. Always report ×g, not RPM, for reproducibility.

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6 / 8

RCF → RPM Calculator (pelleting bacteria)

Convert a target relative centrifugal force (×g) into the rotor speed (RPM) you must dial in for pelleting bacteria, from your rotor radius.

Open this free tool →
7 / 8

RCF → RPM Calculator (DNA ethanol precipitation)

Convert a target relative centrifugal force (×g) into the rotor speed (RPM) you must dial in for DNA ethanol precipitation, from your rotor radius.

Open this free tool →
8 / 8

RPM → RCF Calculator (a plasmid miniprep spin)

Convert centrifuge speed (RPM) to relative centrifugal force (×g) for a plasmid miniprep spin, using your rotor radius. Always report ×g, not RPM, for reproducibility.

Open this free tool →

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