A = εcl in plain language — read concentration straight off a spectrophotometer using the right extinction coefficient.
Use the Beer–Lambert law to convert an absorbance reading of NADH (340 nm) into molar concentration, using its extinction coefficient (6,220 M⁻¹cm⁻¹).
Use the Beer–Lambert law to convert an absorbance reading of NADPH (340 nm) into molar concentration, using its extinction coefficient (6,220 M⁻¹cm⁻¹).
Use the Beer–Lambert law to convert an absorbance reading of TNB / DTNB (412 nm) into molar concentration, using its extinction coefficient (14,150 M⁻¹cm⁻¹).
Use the Beer–Lambert law to convert an absorbance reading of p-Nitrophenol (405 nm) into molar concentration, using its extinction coefficient (18,000 M⁻¹cm⁻¹).
Use the Beer–Lambert law to convert an absorbance reading of o-Nitrophenol (420 nm) into molar concentration, using its extinction coefficient (4,500 M⁻¹cm⁻¹).
Use the Beer–Lambert law to convert an absorbance reading of ABTS radical (414 nm) into molar concentration, using its extinction coefficient (36,000 M⁻¹cm⁻¹).
Convert an A₂₆₀ absorbance reading into the concentration (ng/µL and molar) of a ssdna oligo using its sequence-derived extinction coefficient.
Convert an A₂₆₀ absorbance reading into the concentration (ng/µL and molar) of a pcr primer using its sequence-derived extinction coefficient.
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