ssDNA Oligo Concentration from A₂₆₀ Calculator

Convert an A₂₆₀ absorbance reading into the concentration (ng/µL and molar) of a ssdna oligo using its sequence-derived extinction coefficient.

c = A₂₆₀ / ε (Beer–Lambert, l=1 cm)

Sequence (5′→3′)A₂₆₀ reading

15.298ng/µL

Oligo concentration

2.2075 × 10^-6 M

Molar

226,500

ε (per-nt)

1
Summed extinction coefficient (per-nt approximation)
ε ≈ 226,500 M⁻¹cm⁻¹
2
Beer–Lambert (l = 1 cm)
c = A260·d/ε = 2.208e-6 mol/L
3
Mass concentration (MW ≈ length × 330 g/mol; g/L → ng/µL × 1000)
≈ 15.3 ng/µL

Resuspend and quantify your ssdna oligo accurately from its A₂₆₀.

🔒 100% client-side — your data is computed in the browser and never uploaded.

Cite this tool

ToolJolt. ssDNA Oligo Concentration from A₂₆₀ Calculator. ToolJolt Chemistry & Lab Tools; 2026. https://tooljolt.com

ssDNA Oligo Concentration from A₂₆₀ Calculator for molecular biologists, geneticists and bioinformaticians. Enter your values and read a sourced, step-by-step result instantly, right in your browser.

About ssDNA Oligo Concentration from A₂₆₀ Calculator

Convert an A₂₆₀ absorbance reading into the concentration (ng/µL and molar) of a ssdna oligo using its sequence-derived extinction coefficient. The calculation uses c = A₂₆₀ / ε (Beer–Lambert, l=1 cm). Why accuracy here pays off: Primer design, GC content and melting temperature decide whether a PCR amplifies cleanly or produces nothing. Small sequence mistakes propagate into failed experiments and wasted reagents. Resuspend and quantify your ssdna oligo accurately from its A₂₆₀. Mistakes that trip people up: reading the wrong strand or frame; mismatched forward/reverse primer Tm; primers with runs of G/C causing mispriming. No account, no upload, no tracking of your inputs — the result is generated on your machine, which makes it reproducible, private and citable in published work.

How to use ssDNA Oligo Concentration from A₂₆₀ Calculator

1Enter your input values.
2Read the headline result and the supporting figures, which recompute as you type.
3Open “Worked example with your numbers” to see the substituted formula step by step.
4Copy the result, or use the cite-this-tool snippet for your methods section.

Why use ssDNA Oligo Concentration from A₂₆₀ Calculator?

✓Mobile-friendly and completely free, with no sign-up or usage caps
✓Built on a sourced, unit-tested formula for nucleic-acid sequence analysis
✓Links to related nucleic-acid sequence analysis calculators so you can finish the whole workflow
✓Copy-ready result and a one-line “cite this tool” snippet for your methods section
✓Designed for molecular biologists, geneticists and bioinformaticians who need a trustworthy answer fast

Frequently asked questions

Any tips specific to this calculation?+

Resuspend and quantify your ssdna oligo accurately from its A₂₆₀. Also watch out for: reading the wrong strand or frame and ignoring secondary structure in GC-rich templates.

Is this ssdna oligo concentration from a₂₆₀ calculator free to use?+

Yes. It is completely free, needs no sign-up, and runs entirely in your browser — there are no usage limits.

What formula does it use?+

It uses c = A₂₆₀ / ε (Beer–Lambert, l=1 cm) The full worked example is shown beneath the result so you can verify each step.

What are the most common mistakes here?+

In nucleic-acid sequence analysis, watch for: mismatched forward/reverse primer Tm; primers with runs of G/C causing mispriming; ignoring secondary structure in GC-rich templates; reading the wrong strand or frame. This tool shows the working so you can catch these before they cost an experiment.

Does my data leave my device?+

No. All computation happens locally in your browser. Nothing you enter — sequences, concentrations or measurements — is uploaded to any server, so it is safe for confidential work.

Can I cite this tool?+

Yes — use the “Cite this tool” snippet on the page. Many users link these calculators from methods sections, lab SOPs and teaching materials.

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